Abstract: | Carbon-13 NMR signals were assigned for the complete set of the 26 theoretically possible isomers of methyl 5β-cholanates having one to three hydroxy groups at positions C-3, C-7 and/or C-12 in the nucleus. Substituent effects on the 13C NMR shielding data serving to characterize the position and configuration of the hydroxy groups are discussed. |