| 柱前衍生-反相高效液相色谱法测定荔枝果蒂中的氨基酸 |
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| 引用本文： | 周雪晴,谢艳丽,赵振东,冯玉红.柱前衍生-反相高效液相色谱法测定荔枝果蒂中的氨基酸[J].化学分析计量,2012,21(5):22-24. |
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| 作者姓名： | 周雪晴 谢艳丽 赵振东 冯玉红 |
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| 作者单位： | 海南省精细化工重点实验室,海南大学分析测试中心,海口570228 |
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| 基金项目： | 海南大学2010年青年基金项目（qnjj1003）;海南大学研究生创新平台科研项目基金 |
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| 摘 要： | 建立了AccQ· Tag柱前衍生-高效液相色谱法测定荔枝果蒂中氨基酸含量的方法.荔枝果蒂样品在120℃下真空水解22 h,再与AQC衍生剂进行衍生,并采用反相液相色谱法-荧光检测器进行分析,外标法计算样品中17种氨基酸的含量.17种氨基酸在35 min内可完全分离,荔枝果蒂中氨基酸的含量在2.5～25 μmol/L范围内与色谱峰面积呈良好的线性关系,相关系数不小于0.999.方法的加标回收率在71.2％～92.0％之间,测定结果的相对标准偏差为3.16％～9.94％(n=6),方法的检出限(S/N=3)在0.000 5～0.0012 mg/L之间.
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| 关 键 词： | 柱前衍生 液相色谱法 荔枝果蒂 氨基酸 |
Determination of Amino Acids in Pedicel of Litchi Chinensis by AccQ.Tag-High Performance Liquid Chromatography |
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| Zhou Xueqing
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Xie Yanli
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Zhao Zhendong
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Feng Yuhong.Determination of Amino Acids in Pedicel of Litchi Chinensis by AccQ.Tag-High Performance Liquid Chromatography[J].Chemical Analysis And Meterage,2012,21(5):22-24. |
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| Authors: | Zhou Xueqing Xie Yanli Zhao Zhendong Feng Yuhong |
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| Institution: | (Hainan Provincial Key Lab of Fine Chem, Chemical Analytical and Testing Center of Hainan University, Haikou 570228, China) |
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| Abstract: | A method for the determination of 17 amino acids in pedicel of Litchi Chinensis was established. The amino acids in pedicel ofLitchi Chinensis were hydrolyzed at 120℃ in the vacuum for 22 h, then the hydrolyte were derivated by AQC reagent and determined by HPLC-FLD. The 17 amino acids were separately within 35 min. The content of amino acids in pedicel of Litchi Chinensis had good relationship in the range of 2.5-25 μ mol / L, the correlation coefficient was not less than 0.999. The recoveries were in the range of 71.2%-92.0%, and the relative standard deviations of determination results were 3.16%-9.94%（n=6）. The detection limits （S/N=3） were 0.000 5-0.001 2 mg/L. |
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| Keywords: | precolumn derivatization high performance liquid chromatography pedicel of litchi chinensis aminoacids |
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