Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography |
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| Authors: | G. Odham A. Tunlid L. Larsson P. -A. Mårdh |
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| Affiliation: | (1) Laboratory of Ecological Chemistry, University of Lund, Ecology Building, Helgonavägen 5, S-223 62 Lund, Sweden;(2) Department of Medical Microbiology, University of Lund, Sölvegatan 23, S-223 62 Lund, Sweden |
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| Abstract: | Summary Applications of quantitative gas chromatography/mass spectrometry (selected ion monitoring) to clinical and ecological microbiology are described. The technique permits determination of selected microbial metabolites and can be used for the rapid diagnosis of, for example, tuberculosis by ion-focusing of specific branch-chain fatty acids. The technique is also used to determine diaminopimelic and muramic acids, and D- and L-amino acids separated by chiral gas chromatography, in the low picogram range. The latter method has the potential to detect and quantitate microbial populations.Presented at the  14th International Symposium on Chromatography London, September, 1982 |
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| Keywords: | Mass spectrometry Selected ion monitoring Fused silica capillary GC Chiral capillary GC Tuberculostearic acid Muramic acid Diaminopimelic acid D- and L-alanine |
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