Amperometric biosensors based on microflow injection system |
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Authors: | Inna Eshkenazi Vered Sacks Tova Neufeld Judith Rishpon |
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Institution: | (1) Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, 69978 Ramat-Aviv, Israel |
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Abstract: | Novel electrochemical cells based on a microflow system combined with amperometric enzyme electrodes were developed and served
for quantitative determination of various compounds, such as organophosphates and lactose. The resulting biosensors are selective
and efficient owing to immobilization of the sensing elements on the electrodes. The sensors are easy to operate, and the
procedures are rapid, accurate, reproducible, and inexpensive, requiring neither special skills and training nor complicated
instrumentation. The use of a microflow cell ensures the continuous flux of a new substrate, thus preventing the accumulation
or adsorption of products to the electrode. Miniaturization of the sensor has two main advantages: (1) it is easy to carry
and therefore can be used outdoors as well, and (2) it allows working with low volumes of compounds and reagents, which is
highly important when dealing with hazardous compounds. |
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Keywords: | Microflow amperometric biosensor injection electrode |
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