Site-directed photochemical disruption of the actin cytoskeleton by actin-binding Rose Bengal-conjugates |
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| Authors: | Conlon Kimberly A Rosenquist Thomas Berrios Miguel |
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| Affiliation: | Department of Pharmacological Sciences, School of Medicine, University Hospital and Medical Center, State University of New York at Stony Brook, Stony Brook, NY 11794-8651, USA. conlon@pharm.sunysb.edu |
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| Abstract: | The in situ light-induced, non-enzymatic digestion of cytoskeletal actin by a xanthene dye conjugated to heavy meromyosin, anti-actin antibodies and/or anti-myosin antibodies is reported. The dye Rose Bengal was conjugated to either anti-actin antibodies, anti-myosin antibodies or heavy meromyosin. Under our experimental conditions, visible light induced the non-enzymatic breakdown of cytoskeletal actin when mammalian tissue culture cells were probed either with Rose Bengal-conjugated anti-actin and/or anti-myosin antibodies. Similar results were obtained when tissue culture cells were probed with Rose Bengal-conjugated heavy meromyosin before irradiation with visible light. The in situ photochemical reaction depended on the presence of actin-binding Rose Bengal-conjugates. |
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