Reversed phase high performance liquid chromatography of glycopeptides |
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| Authors: | T H Wang T Chen D F Barofsky |
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| Affiliation: | Department of Agricultural Chemistry, Oregon State University, Corvallis 97331. |
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| Abstract: | A practical procedure for isolating and purifying glycopeptides is described, viz. enzymatic hydrolysis-gel permeation chromatography-ion exchange chromatography-reversed phase HPLC. Using this procedure 28 glycopeptides from hen ovalbumin have been isolated some of which hitherto have not been identified. Water was a suitable mobile phase for preparing pure glycopeptides, and control of column temperature was important for good separations and reproducible retention times. Structural confirmation was by fast atom bombardment mass spectrometry. |
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