多元曲线分辨-交替最小二乘法应用于延胡索药材指纹图谱中重叠峰分辨及延胡索乙素的含量测定

采用光谱相关色谱法对延胡索药材指纹图谱中的有效组分延胡索乙素峰进行确认；利用多元曲线分辨 交替最小二乘法（MCR-ALS）对延胡索乙素重叠峰簇进行解析，得到延胡索乙素的真实色谱峰，其光谱还原率大于0.999，并对其进行准确定量，该方法可作为指纹图谱的辅助方法，为中药的真伪鉴定和质量客观评价提供可靠依据；最后讨论了药材的炮制对延胡索乙素含量的影响。

The Applications of Multivariate Curve Resolution-alternating Least Squares in Resolving Overlapped Spectra of Corydalis yanhusuo Fingerprint and the Content Detection of Tetrahydropalmatine

Spectral correlative chromatography was used to identify the peak of tetrahydropalmatine in Corydalis yanhusuo fingerprint. Moreover, multivariate curve resolution-alternating least squares(MCR-ALS) was used to resolve the overlapping peaks of tetrahydropalmatine and restore its true peak shape. More than 99.9% spectrum could be recovered and accurately quantitative results were obtained. The method can be used as an auxillary fingerprinting method to provide a reliable basis for the authenticity and quality assessment of Chinese medicines. The effect of the herbs-cooking on the content of tetrahydropalmatine was also discussed.