一步法制备银溶胶用于临床嗜麦芽窄食单胞菌的表面增强拉曼光谱快速检测

该文利用一步法快速制备银溶胶,实现了基底的现用现制。对嗜麦芽窄食单胞菌进行表面增强拉曼散射(SERS)检测,得到18组拉曼特征峰的生物指纹谱图。方法能够检测到的最低菌悬液浓度为5×106菌落形成单位(CFU)/mL,重现性的相对标准偏差为6.8%~16%,满足生物医学领域定性分析的基本要求。采用该方法获得的阳性病人血液中的病原细菌细胞的拉曼信号与纯培养菌株接近,进一步证明这种快速(10 min)、简单、便携式和低成本的方法在临床诊断中具有广阔的应用前景。

Clinical Rapid Detection of Stenotrophomonas Maltophilia Using Surface Enhanced Raman Scattering with One step Method Prepared Silver Colloid

In this study,a silver colloid as surface enhanced Rarman scattering(SERS) substrate was rapidly synthesized via one step method.The biological fingerprint spectra of 18 groups of Raman characteristic peaks were thereafter obtained from the detection of opportunistic pathogen Stenotrophomonas maltophilia by SERS.Results demonstrated that the minimum concentration of bacterial suspension could reach 5×106 colony forming units(CFU)/mL with the relative standard deviations(RSDs) for repeatability detection of 6.8%-16%,which could meet the basic requirements for qualitative analysis in biomedical field.Besides,the Raman signals of pathogenic bacterial cells in the blood of positive patients obtained by this method are close to those of pure cultured strains,further indicating that this method is rapid(10 min),facile,portable and low cost,and has a broad application prospect in clinical diagnosis.