不同类型探针对免疫层析方法的分析灵敏度影响研究

为研究不同类型探针对免疫层析方法的分析灵敏度影响,该研究以17β-雌二醇(17f3-E2)为检测对象,采用磁性纳米材料标记7 μg/mL的17β-E2单克隆抗体(检测抗体)制备了传统探针,采用磁性纳米材料分别标记5 μg/mL的17P-E2单克隆抗体和5 μg/mL的羊抗鼠IgG多克隆抗体(第二抗体)制备了配对探针,采...

Study on Influences of Different Probes on Analytical Sensitivity of Immunochromatography Assay

To study the influences of different probes on the analytical sensitivity of immunochromatography assay(ICA),a traditional probe was prepared for the detection of 17β-estradiol(17β-E2),with magnetic nanoparticles(MNPs)to label 7μg/mL 17β-E2 monoclonal antibody(mAb,detection Ab).Meanwhile,a paired probe was prepared by using MNPs to separately label 5μg/mL 17β-E2 mAb and 5μg/mL goat anti-mouse polyclonal antibody(pAb,the second antibody),and an indirect probe was prepared by using MNPs to label 14μg/mL pAb.Then,three immunochromatography assays were established based on the three probes for the rapid detection of estradiol.The analytical sensitivities of three ICAs were compared by the 17β-E2 detection.Results showed that the analytical sensitivities of ICAs based on traditional,paired and indirect probes for 17β-E2 are 1,0.5,0.2 ng/mL,respectively.Among these three ICAs,the analytical sensitivity of indirect probe based ICA is the highest.Compared with the traditional and paired probes based ICAs,the detection limit of the indirect probe based ICA is 5 fold and 2.5 fold reduced,respectively.This study refreshes the conventional cognition toward the signal probe which is considered can only be prepared by using signal materials to label the detection Ab in the traditional immunochromatography assay,and provides a valuable reference for the rapid detection of other food safety hazard factors by immunochromatography assays.