Toxicity Test n-Hexane: Ethyl Acetate (3:7) Fraction of Sudamala (Artemisia vulgaris L.)

Sudamala (Artemisia vulgaris L.) is commonly used in the community as anti-tumor in digestive organ, including in oral cavity. However, there have been no scientific studies about oral anti-carcinogenic active substances of Sudamala. The purpose of this study was to analyse the effect of per oral administration of n-hexane: ethyl acetate (3:7) fraction of Artemisia vulgaris L. on healthy mice. This experimental study used male Swiss Webster (Balb C) strain mice (Mus musculus) 2.5 months old, 20-30 grams by weight. Group 1, the mice received 0.5% CMC- Na fraction solvent, as a control. Group 2, the mice received 200 mg/kgbw n-hexane: ethyl acetate (3:7) fraction of Sudamala (Artemisia vulgaris L). The fraction was given once daily for 8 weeks per oral according to the mice's weight. At the end of 8^th week, the mice were killed and oral mucosal tissue was taken for biopsy specimens. Tunnel assay for Apoptosis and immunohistochemistry were undertaken for PCNA. T test analysis showed there was no significant difference between control and treatment groups. The fractions of n-hexane: ethyl acetate (3:7) of Sudamala (Artemisia vulgaris L) was not toxic, it was not harmful to living tissue in healthy mice.