| 电穿孔法在量子点标记细胞中的应用 |
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| 引用本文： | 杨蕊,冀伟,闫玉禧,史艳宇,费强,牟颖,金钦汉.电穿孔法在量子点标记细胞中的应用[J].高等学校化学学报,2005,26(6):1043-1045. |
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| 作者姓名： | 杨蕊 冀伟 闫玉禧 史艳宇 费强 牟颖 金钦汉 |
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| 作者单位： | 1. 吉林大学化学学院微分析仪器研究所, 吉林省光谱分析仪器工程技术研究中心, 长春130023;
2. 吉林大学分子酶学工程教育部重点实验室, 长春130023;
3. 吉林大学基础医学院, 长春130021 |
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| 基金项目： | 国家质检总局重大科技攻关项目 |
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| 摘 要： | 量子点是一种新兴的荧光标记物，它具有发射峰窄而对称且重叠小、发射波长可调、无光褪色现象以及生物相容性好等优点。可进行生物活体及细胞的标记和检测，用量子点标记细胞的方法目前已有较多报道，其中以利用细胞内吞作用的居多，但这些方法需要在量子点上连接具有穿膜功能的生物试剂，而这又可能导致量子点的聚集以及荧光强度的下降乃至消失，
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| 关 键 词： | 量子点 细胞标记 电穿孔 |
| 文章编号： | 0251-0790(2005)06-1043-03 |
| 收稿时间： | 2004-12-21 |
Tagging Cells with Quantum Dots by Electroporation |
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| YANG Rui,JI Wei,YAN Yu-xi,SHI Yan-Yu,FEI Qiang,MU Ying,JIN Qin-Han.Tagging Cells with Quantum Dots by Electroporation[J].Chemical Research In Chinese Universities,2005,26(6):1043-1045. |
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| Authors: | YANG Rui JI Wei YAN Yu-xi SHI Yan-Yu FEI Qiang MU Ying JIN Qin-Han |
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| Institution: | 1. Institute for Miniature Instrumentation, College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Instruments, Jinlin University, Changchun 130023, China;
2. Key Laboratory for Molecular Enzymology Engineering of Ministry of Education, Jilin University, Changchun 130023, China;
3. College of Basic Medicine, Jilin University, Changchun 130021, China |
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| Abstract: | An electroporation method was proposed to make QDs pass through the membranes of K562 leukaemia cells. This method is operated simply and facilitates the tagging experiment greatly. The results show that when the electroporation voltage is 200 V and the lasting time is 1 ms, a high tagging rate is reached. A lot of cells can be tagged at the same time and the livability of the cells is 60%. The whole process can be completed in 20 min. This method brings forward some more extrusive advantages than cell endocytosis and microinjection method, and there should have a very good foreground for further applications. |
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| Keywords: | Quantum dot Cell tagging Electroporation |
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