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Modified screen-printed electrodes for the investigation of the interaction of non-electroactive quinazoline derivatives with DNA |
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| Authors: | J Labuda M Bučková S Jantová I Štepánek I Surugiu B Danielsson M Mascini |
| Institution: | Department of Analytical Chemistry, Slovak Technical University, SK-81237 Bratislava, Slovakia, e-mail: labuda@chtf.stuba.sk, XX Department of Microbiology, Biochemistry and Biology, Slovak Technical University, SK-81237 Bratislava, Slovakia, XX FACH, SK-080 06 Pre?ov, Slovakia, XX Department of Pure and Applied Biochemistry, Lund University, S-22100 Lund, Sweden, SE Department of Public Health, Environmental and Analytical Chemistry, University of Firenze, I-50121 Firenze, Italy, IT |
| Abstract: | Five morpholino-quinazoline derivatives have been investigated voltammetrically using a competition with the tris(o-phenanthroline) cobalt(III) redox marker for the accumulation at dsDNA modified screen-printed electrodes. An association of quinazolines with DNA was observed at the modified electrodes polarized by the negative potential of –0.4 V vs. Ag/AgCl. This was confirmed by a potentiometric stripping analysis based on the DNA guanine signal. Calibration curves for quinazolines within a concentration range of μmol/L were obtained with DP voltammetry using 5 × 10–7 mol/L Co(phen)3 3+ marker. The quinazolines exhibit no effect on the DNA complex with the fluorescent thiazole orange derivative TO-PRO-3. The role of the accumulation potential in the association interaction with DNA is discussed. |
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