Nano-electrospray ionization mass spectrometry: addressing analytical problems beyond routine |
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Authors: | M. Karas U. Bahr T. Dülcks |
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Affiliation: | Instrumental Analytical Chemistry, JW Goethe-University, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany, DE
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Abstract: | The advent of nano-electrospray ionization (nano-ESI) has considerably extended the usability of ESI in the analytical mass spectrometric laboratory. One of the remarkable features of nano-ESI is its extremely low sample consumption. Only a few microliters of analyte solution (10–5–10–8 M) are sufficient for molecular weight determination and structural investigations by MS/MS. But nano-ESI is more than just a minimized-flow ESI; the low solvent flow rate also affects the mechanism of ion formation. As a consequence, the area of ESI-MS applications is significantly enhanced. Oligosaccharides, glycosides as well as glycoproteins can be analyzed more easily than with normal ion spray. The same holds for the analysis of non-covalent complexes sprayed directly from aqueous solutions. |
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