PowerSlicing to determine fluorescence lifetimes of water-soluble organic matter derived from soils,plant biomass,and animal manures |
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Authors: | Tsutomu Ohno Zheming Wang Rasmus Bro |
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Institution: | (1) Department of Plant, Soil, and Environmental Sciences, University of Maine, 5722 Deering Hall, Orono, ME 04469–5722, USA;(2) Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, 902 Battelle Blvd, Mail Stop K8–96, Richland, WA 99352, USA;(3) Faculty of Life Sciences, Department of Food Science, University of Copenhagen, Rolighedsvej 30, 1958 Frederiksberg C, Denmark |
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Abstract: | Time-resolved fluorescence spectroscopy was used to characterize water-soluble organic matter (WSOM) which plays an important
role in soil ecosystem processes. WSOM was extracted from plant biomass, animal manures, and soils from controlled cropping
systems studies with known histories of organic amendments. Lifetime constants were derived using the multi-way PowerSlicing
method which provides a non-iterative, multi-exponential fitting of decay profiles. The lifetimes obtained by PowerSlicing
were not significantly different from those obtained using the traditional discrete components analysis. The three components
attributed to WSOM had lifetimes of 0.38 ± 0.14, 2.11 ± 0.72, and 7.08 ± 1.18 ns which are in agreement with previous lifetimes
reported for humic substances. This study provides further support for the new paradigm for the structure of soil organic
matter where the organic matter is composed of low-molecular-weight components held together by hydrogen bonding and hydrophobic
interactions. |
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Keywords: | Fluorescence spectroscopy Dissolved organic matter Lifetimes Parallel factor analysis |
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