| 烟酰胺与DNA相互作用的电化学研究 |
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| 引用本文： | 徐慧,牟保畏,吕菊波,柳全文.烟酰胺与DNA相互作用的电化学研究[J].化学通报,2009,72(6). |
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| 作者姓名： | 徐慧 牟保畏 吕菊波 柳全文 |
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| 作者单位： | 鲁东大学化学与材料科学学院,烟台,264025
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| 基金项目： | 鲁东大学博士科研启动基金 |
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| 摘 要： | 利用示差脉冲伏安法研究了烟酰胺(NA)与小牛胸腺DNA在pH 8.0条件下相互作用的电化学行为.双链DNA(dsDNA)或单链DNA(ssDNA)的存在导致NA的峰电流明显降低且峰电位负移,表明NA与DNA发生相互作用,生成了复合物,且其作用模式主要是静电模式,但NA与dsDNA的相互作用强于与ssDNA的相互作用,可用于识别dsDNA和ssDNA.通过dsDNA加入前后峰电流的变化,计算得出NA与dsDNA结合常数β=4.946×10(11),结合位点数m=3.此外,NA的峰电流Ip与DNA质量浓度在1～14mg/L的范围内呈线性关系,线性回归方程为Ip(10-5A)=-0.03451cDNA(mg/L)+1.7408,相关系数R为0.9998.该法具有良好的回收率和选择性,可用于样品中DNA的测定.
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| 关 键 词： | 烟酰胺 小牛胸腺DNA 相互作用 电化学 |
Electrochemical Investigation on the Interaction between Nicotinamide and DNA |
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| Xu Hui,Mou Baowei,Lv Jubo,Liu Quanwen.Electrochemical Investigation on the Interaction between Nicotinamide and DNA[J].Chemistry,2009,72(6). |
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| Authors: | Xu Hui Mou Baowei Lv Jubo Liu Quanwen |
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| Institution: | School of Chemistry and Materials Science;Ludong University;Yantai 264025 |
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| Abstract: | The electrochemical behavior of the interaction of nicotinamide (NA) and DNA at pH 8.0 was investigated by differential pulse voltammetry. After the addition of the dsDNA or ssDNA,the peak currents declined with the peak potential of NA shifting negatively. The results showed that a complex was formed due to the interaction of NA and DNA,and electrostatic attraction existed between NA and dsDNA or ssDNA. The interaction between NA and dsDNA was more strongly than that with ssDNA,by which dsDNA and ssDNA cou... |
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| Keywords: | Nicotinamide DNA Interaction Electrochemistry |
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