| 灰色链霉菌ATCC 13273 位点特异性转化商陆皂苷元羟基化反应研究 |
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| 引用本文： | 钱利武a,张剑a,刘吉华b,余伯阳b.灰色链霉菌ATCC 13273 位点特异性转化商陆皂苷元羟基化反应研究[J].中国化学,2009,27(7):1434-1436. |
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| 作者姓名： | 钱利武a 张剑a 刘吉华b 余伯阳b |
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| 作者单位： | （a 中国药科大学中药复方研究室，江苏，南京 210038 ;b 中国药科大学现代中药教育部重点实验室，江苏，南京 210038） ; |
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| 摘 要： | 为增加商陆皂苷元的结构多样性，本研究采用两步培养法，利用灰色链霉菌ATCC 13273对其进行生物转化，得到一个极性增大的羟基化产物。通过二维核磁、高分辨质谱等方法鉴定该转化产物为2β, 3β, 23, 29-四羟基-齐墩果烷-12-烯-28, 30-二酸-30-甲酯。
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| 关 键 词： | 生物转化 商陆皂苷元 灰色链霉菌 微生物羟基化 五环三萜 |
| 收稿时间： | 2008-12-3 |
| 修稿时间： | 2009-1-20 |
Regio‐specific Microbial Hydroxylation of Phytolaccagenin by Streptomyces griseus ATCC 13273 |
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| Liwu QIAN,Jian ZHANG,Jihua LIU,Boyang YU.Regio‐specific Microbial Hydroxylation of Phytolaccagenin by Streptomyces griseus ATCC 13273[J].Chinese Journal of Chemistry,2009,27(7):1434-1436. |
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| Authors: | Liwu QIAN Jian ZHANG Jihua LIU Boyang YU |
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| Abstract: | Microbial transformation of one oleane‐type pentacyclic triterpene aglycone, phytolaccagenin (2β,3β,23‐trihydroxy‐olean‐12‐ene‐28,30‐dioic acid 30‐methyl ester) by Streptomyces griseus ATCC 13273 was investigated for developing new bioactive derivatives. A new oxidized metabolite, through the regio‐specific hydroxylation on the C‐29 methyl group, was obtained from the preparative‐scale biotransformation with a standard two‐stage fermentation protocol. The metabolite was identified as 2β,3β,23,29‐tetrahydroxy‐olean‐12‐ene‐28,30‐dioic acid 30‐methyl ester by mass and 2D‐NMR spectra. |
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| Keywords: | biotransformation phytolaccagenin Streptomyces griseus microbial hydroxylation pentacyclic triterpene |
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