天麻光谱性质及其与牛血清白蛋白作用的研究

用荧光光谱法和紫外吸收光谱法研究了中药天麻(GE)的光谱性质,以及GE与牛血清白蛋白(BSA)的相互作用。考察了溶剂、煮沸时间和pH值等影响因素对GE荧光性质的影响。实验确定用水提取液煮沸10min配成储备液,在中性条件下进行测定。发现了BSA的荧光被猝灭和GE的荧光被敏化,从而说明二者之间存在能量转移和结合作用。在BSA与GE的作用过程中,BSA中酪氨酸的构象发生了改变。

The Spectral Properties of Gastrodia Elata and the Interaction between Gastrodia Elata and Bovine Serum Albumin

The spectral properties of Chinese traditional medicine Gastrodia Elata(GE), and the interaction between GE and bovine serum albumin(BSA) were studied with fluorescence and UV-Vis absorption spectroscopy. The spectral properties of GE in different conditions were investigated, such as solvent, boiling times and pH. The fluorescence quenching of BSA was observed accompanying the fluorescence sensitization of GE, which suggested that energy transfer and combination between BSA and GE occured. In the process of the interaction between BSA and GE, the conformation of the tyrosine residue in BSA changed.