| 聚甲基戊烯膜式氧合器表面碳酸酐酶的固定化及性能研究 |
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| 引用本文： | 王琴梅,张涤华,廖艳红,张静夏,廖志群.聚甲基戊烯膜式氧合器表面碳酸酐酶的固定化及性能研究[J].化学通报,2009,72(6). |
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| 作者姓名： | 王琴梅 张涤华 廖艳红 张静夏 廖志群 |
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| 作者单位： | 王琴梅,Wang Qinmei(中山大学附属一院,广州,510080;中山大学心血管研究所,广州,510080);张涤华,廖艳红,Zhang Dihua,Liao Yanhong(中山大学附属一院,广州,510080);张静夏,Zhang Jingxia(中山大学药学院,广州,510080);廖志群,Liao Zhiqun(中山大学公共卫生学院,广州,510080)
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | 聚甲基戊烯(PMP)膜式氧合器表面先用水等离子体改性,以引入羟基官能团;再以溴化氰为偶联剂,将碳酸酐酶(CA)偶联至其表面.改性后用X射线光电子能谱(XPS)、表面接触角及酶活性测定等方法研究PMP表面性能变化.结果表明,等离子体处理后,在PMP表面引入了大量的含氧官能团,与水的表面接触角从103.37°降低至50.01°.再将CA引入PMP表面后,与水的表面接触角进一步降低至39.23°;XPS的C1谱图中出现蛋白质的特征峰;以对硝基苯酚乙酸酯为底物,测得表面接枝CA的活性达到理论单分子层接枝活性的73%.改性后PMP表面物理化学性质的变化证明本文方法确实能成功地将羟基官能团、CA引入其表面.本方法有望应用在膜式氧合器上以提高其清除血液中CO2的能力.
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| 关 键 词： | 聚甲基戊烯 碳酸酐酶 X射线光电子能谱 表面接触角 水等离子体 |
Immobilization and Characterization of Carbonic Anhydrase on the Surface of Polymethylpentene Membrane Oxygenator |
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| Wang Qinmei ,Zhang Dihua,Liao Yanhong ,Zhang Jingxia,Liao Zhiqun.Immobilization and Characterization of Carbonic Anhydrase on the Surface of Polymethylpentene Membrane Oxygenator[J].Chemistry,2009,72(6). |
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| Authors: | Wang Qinmei Zhang Dihua Liao Yanhong Zhang Jingxia Liao Zhiqun |
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| Institution: | 1 First Affiliated Hospital;2 Institute of Cardiology and Angiology;3 School of Medicine;4 School of Public Health;Sun Yat-sen University;Guangzhou 510080 |
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| Abstract: | The surface of polymethylpentene (PMP) membrane oxygenator was treated by water plasma and then carbonic anhydrase (CA) was introduced onto its surface by the crosslinker of cyanate bromide (CNBr). The character of PMP surface was studied by analysis of X-ray photoelectron spectroscopy (XPS),measurements of surface contact angle and enzyme activity after modification. The results showed that,after water plasma treatment,large oxygen containing groups were formed on PMP surface,the surface contact angle with... |
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| Keywords: | Polymethylpentene Carbonic anhydrase X-ray photoelectron spectroscopy Surface contact angle Water plasma |
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