A comparison of Tris-glycine and Tris-tricine buffers for the electrophoretic separation of major serum proteins |
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Authors: | Haider Syed R Sharp Barry L Reid Helen J |
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Institution: | Centre for Analytical Science, Department of Chemistry, Loughborough University, Leicestershire, UK. |
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Abstract: | This paper compares different buffer systems for the electrophoretic separation of the five most abundant serum proteins on native-PAGE gel and cellulose membranes. A modified Tris-tricine system was shown to be superior for the separation of these serum proteins in a 7% m/v native-PAGE gel as compared with the traditionally used Tris-glycine and Tris-tricine methods. This modified Tris-tricine buffer system was also employed for the separation of serum proteins using a cellulose acetate membrane and very effective separation was observed as compared with the traditionally used Tris-barbital and Tris-glycine buffer systems. |
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Keywords: | Native‐PAGE SDS‐PAGE Serum proteins Tris‐glycine Tris‐tricine |
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