An electron microscopic study of the archaeal feast/famine regulatory protein: 6. The cylindrical assembly form |
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| Authors: | Sanae A Ishijima Lester Clowney Masashi Suzuki |
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| Institution: | *)National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Center 6-10, Higashi, 1-1-1, Tsukuba, Ibaraki 305-8566, Japan; **)Japan Science and Technology Agency (JST), Core Research for Evolutionary Science and Technology (CREST), Honmachi, 4-1-18, Kawaguchi Center Building, Kawaguchi, Saitama 332-0012, Japan |
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| Abstract: | Using a microcrystal of the feast/famine regulatory protein (FFRP) pot0434017 (FL11), a cryo-electron micrograph was taken, showing a projection of cylinder-like assemblies packed parallel to each other. This electron micrograph was processed in the Fourier space by selecting spots reflecting the packing and, in addition, those reflecting stacking of units inside the cylinders. Twenty seven subimages were selected, each containing three cylinders of 24 discs each, running nearly parallel to each other. By averaging these images and in combination with another average showing a different view Ishijima, S. A., Clowney, L., and Suzuki, M. (2004) Proc. Jpn. Acad., Ser. B 80, 183–188], some details of the 3D structure of the cylindrical assembly form are discussed. |
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