Selective glucose sensing in complex media using a biomimetic receptor |
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Authors: | Robert A. Tromans Soumen K. Samanta Andy M. Chapman Anthony P. Davis |
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Affiliation: | School of Chemistry, University of Bristol, Cantock''s Close, Bristol BS8 1TS UK.; Carbometrics Ltd., Unit DX, St Philips Central, Albert Road, Bristol BS2 0XJ UK |
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Abstract: | Glucose is a key biomedical analyte, especially relevant to the management of diabetes. Current methods for glucose determination rely on the enzyme glucose oxidase, requiring specialist instrumentation and suffering from redox-active interferents. In a new approach, a powerful and highly selective achiral glucose receptor is mixed with a sample, l-glucose is added, and the induced CD spectrum is measured. The CD signal results from competition between the enantiomers, and is used to determine the d-glucose content. The involvement of l-glucose doubles the signal range from the CD spectrometer and allows sensitivity to be adjusted over a wide dynamic range. It also negates medium effects, which must be equal for both enantiomers. The method has been demonstrated with human serum, pre-filtered to remove proteins, giving results which closely match the standard biochemical procedures, as well as a cell culture medium and a beer sample containing high (70 mM) and low (0.4 mM) glucose concentrations respectively.A highly selective receptor, circular dichroism and chiral competition are combined in this versatile method for d-glucose analysis. |
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