Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
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| Authors: | Raja Chouket,Agnè s Pellissier-Tanon,Annie Lemarchand,Agathe Espagne,Thomas Le Saux,Ludovic Jullien |
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| Affiliation: | PASTEUR, Département de Chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 24, rue Lhomond, 75005 Paris France, +33 4432 3333 ; Sorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), 4 Place Jussieu, Case Courrier 121, 75252 Paris Cedex 05 France |
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| Abstract: | Interrogating living cells requires sensitive imaging of a large number of components in real time. The state-of-the-art of multiplexed imaging is usually limited to a few components. This review reports on the promise and the challenges of dynamic contrast to overcome this limitation.Interrogating living cells requires sensitive imaging of a large number of components in real time. |
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