Spectroscopic evidence for the formation of goldfingers |
| |
Authors: | Franzman Matthew A Barrios Amy M |
| |
Institution: | Department of Chemistry, University of Southern California, Los Angeles, California 90089, USA. |
| |
Abstract: | Gold(I) has long been used in the treatment of rheumatoid arthritis, but the therapeutically relevant biological targets of gold(I) are not well understood. Here, we report the results of a spectroscopic investigation into the formation of goldfingers. By exploiting a thiolate to gold charge-transfer band in the UV, we observed that gold(I) interacts with zinc finger peptides with a stoichiometry of one gold ion for each two cysteine residues, forming 1:1, 1.5:1, and 2:1 adducts with zinc finger peptides containing CCHH, CCHC, and CCCC donor sets, respectively. In addition, circular dichroism experiments provided evidence that goldfingers are more ordered than the corresponding metal-free peptides but do not exhibit the canonical zinc finger structure. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|