Separation behavior of electron-beam curing derived, acrylate-based monoliths |
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Authors: | Bandari Rajendar Elsner Christian Knolle Wolfgang Kühnel Christa Decker Ulrich Buchmeiser Michael R |
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Institution: | Leibniz-Institut für Oberfl?chenmodifizierung IOM, Leipzig, Germany. |
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Abstract: | Electron beam (EB) curing-derived monolith materials were prepared from ethyl methacrylate (EMA), trimethylolpropane triacrylate (TMPTA), 2-propanol, 1-dodecanol, and toluene within the confines of 3 mmx100 mm id glass columns, applying a total dose of 22 kGy for curing. Monolithic columns were checked for their separation behavior for selected dansylated (DNS)-amino acids as well as for cyclophilin 18. Their separation performance was compared to that of a C18-modified silica-based rigid rod (Chromoliths). In the separation of dansylated amino acids, retention times were reduced on EB-derived columns, where the peak resolution was significantly better than on a Chromolith. This finding was attributed to a larger fraction of small pores (<2.15 nm) in the EB curing-derived monoliths. Finally, EB curing-derived monoliths have been used to separate cyclophilin 18 from crude cell lysis mixtures. |
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