| 对巯基苯胺在拉曼免疫检测中的应用 |
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| 引用本文： | 蒋芸,崔颜,顾仁敖.对巯基苯胺在拉曼免疫检测中的应用[J].光谱学与光谱分析,2006,26(7):1260-1263. |
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| 作者姓名： | 蒋芸 崔颜 顾仁敖 |
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| 作者单位： | 苏州大学化学系,江苏,苏州,215006;苏州大学化学系,江苏,苏州,215006;苏州大学化学系,江苏,苏州,215006 |
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| 基金项目： | 国家自然科学基金
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国家重点实验室基金
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高等学校博士学科点专项科研项目 |
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| 摘 要： | 文章以对巯基苯胺(PATP)作为标记分子,研究其在金电极上的吸附.发现对巯基苯胺分子是采用苯环直立于电极表面的吸附方式吸附于电极表面的.用于免疫检测时,对巯基苯胺分子先通过直立吸附的方法达到满单层吸附,然后通过胺基与抗体分子的羧基相连,形成一种复层结构,再与基底结合,得到固相抗体-抗原-标记抗体"三明治"结构.借助抗体上标记金纳米粒子的SERS信号达到单组分生物免疫检测的目的.
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| 关 键 词： | 表面增强拉曼光谱(SERS) 对巯基苯胺 金纳米粒子 免疫检测 |
| 文章编号： | 1000-0593（2006）07-1260-04 |
| 收稿时间： | 2005-05-28 |
| 修稿时间： | 2005-08-28 |
Application of p-Aminothiophenol as a SERS Reporter in Immunoassay |
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| JIANG Yun,CUI Yan,GU Ren-ao.Application of p-Aminothiophenol as a SERS Reporter in Immunoassay[J].Spectroscopy and Spectral Analysis,2006,26(7):1260-1263. |
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| Authors: | JIANG Yun CUI Yan GU Ren-ao |
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| Institution: | Department of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China |
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| Abstract: | The adsorption of p-aminothiophenol(PATP) molecule on the gold electrode was investigated.The result suggests that PATP was adsorbed perpendicularly onto the substrate.By immobilizing goat anti-mouse IgG on a glass slide modified by special organic molecules,the antibody/antigen/reporter-labeled immunogold nanoparticle sandwich assay was build for surface-enhanced Raman spectroscopy(SERS) measurements.The p-aminothiophenol(PATP) was used as SERS-reporter molecule.Thus the authors can identify the single type biospecific species by SERS signal of the reporter molecules which are coimmobilized with antibody molecules in gold colloids. |
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| Keywords: | Surface-enhanced Raman spectroscopy(SERS) p-aminothiophenol(PATP) Gold nanoparticle Immunoassay |
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