ROLE OF COMPLEX FORMATION IN THE PHOTOSENSITIZED DEGRADATION OF DNA INDUCED BY N'-FORMYLKYNURENINE |
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Authors: | P Walrant R Santos M Charlier |
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Institution: | Laboratoire de Biophysique Muséum National d'Histoire Naturelle, 61, Rue Buffon 75005 Paris, France and Centre de Biophysique Moléculaire, 45045 Orleans Cedex, France |
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Abstract: | Abstract— N'-Formylkynurenine derivatives efficiently bind to DNA or polynucleotides. Homopolynucleotides and DNA display marked differences in the binding process. Association constants are derived which indicate that the oxidized indole ring is more strongly bound to DNA than the unoxidized one. Irradiation of such complexes with wavelengths greater than 320 nm induces pyrimidine dimer formation as well as DNA chain breaks. Complex formation is shown to play an important role in these photosensitized reactions. The photodynamic action of N'-formylkynurenine on DNA constituents is negligible at neutral pH but guanine and xanthine derivatives are sensitizable at higher pH. Thymine dimer splitting can occur in aggregated frozen aqueous solutions of N'-formylkynurenine and thymine dimer but this photosensitized splitting is negligible in liquid solutions at room temperature. |
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