Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter |
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Authors: | Wu Ting-Hsiang Chen Yue Park Sung-Yong Hong Jason Teslaa Tara Zhong Jiang F Di Carlo Dino Teitell Michael A Chiou Pei-Yu |
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Institution: | Department of Mechanical and Aerospace Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, Rm 37-138, Los Angeles, CA 90095, USA. |
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Abstract: | We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20,000 mammalian cells s(-1) with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1500 cells s(-1) or 3000 beads s(-1). Fast switching (30 μs) and a small perturbation volume (~90 pL) is achieved by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel. |
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