Chemiluminescent Detection of Carbohydrates in the Tumoral Breast Diseases |
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Authors: | Vanessa Passos Brustein Carmelita Lima Bezerra Cavalcanti Mario Ribeiro de Melo-Junior Maria Tereza Santos Correia Eduardo Isidoro Carneiro Beltrão Jr" target="_blank">Luiz Bezerra CarvalhoJr |
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Institution: | 1.Laboratório de Imunopatologia Keizo Asami,LIKA/UFPE,Recife,Brazil;2.Departamento de Química Fundamental,DQF/UFPE,Recife,Brazil;3.Departamento de Patologia,CCS/UFPE,Recife,Brazil;4.Departamento de Bioquímica,CCB/UFPE,Recife,Brazil |
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Abstract: | Nowadays, there is an increase of investigations into the fibroadenoma, mainly because some studies have shown that the occurrence
of fibroadenoma is linked to an increased risk of developing breast carcinoma. Currently, the chemiluminescence biomarkers
are applied for validation methods and screening. Here, a lectin chemiluminescence is proposed as new histochemistry method
to identify carbohydrates in mammary tumoral tissues. The lectins concanavalin A (Con A) and peanut agglutinin (PNA) conjugated
to acridinium ester were used to characterize the glycocode of breast tissues: normal, fibroadenoma, and invasive duct carcinoma
(IDC). The lectin chemiluminescence expressed in relative light units (RLU) was higher in fibroadenoma and IDC than in normal
tissue for both lectins tested. The relationship RLU emission versus tissue area described a linear and hyperbolic curve for
IDC and fibroadenoma, respectively, using Con A whereas hyperbolic curves for both transformed tissues using PNA. RLU was
abolished by inhibiting the interaction between tissues and lectins using their specific carbohydrates: methyl-α-d-mannoside (Con A) and galactose (PNA). The intrinsic fluorescence emission did not change with combination of the lectins
(Con A/PNA) to the acridinium ester for hydrophobic residues. These results represent the lectin chemiluminescence as an alternative
of histochemistry method for tumoral diagnosis in the breast. |
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