Optimization of Laccase Production by <Emphasis Type="Italic">Trametes versicolor</Emphasis> Cultivated on Industrial Waste |
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| Authors: | Marina Ti?ma Polona ?nidar?i?-Plazl ?ur?a Vasi?-Ra?ki Bruno Zeli? |
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| Institution: | (1) Faculty of Food Technology Osijek, J. J. Strossmayer University of Osijek, F. Kuhača 18, 31000 Osijek, Croatia;(2) Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia;(3) Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia; |
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| Abstract: | Laccases are very interesting biocatalysts for several industrial applications. Its production by different white-rot fungi
can be stimulated by a variety of inducing substrates, and the use of lignocellulosic wastes or industrial by-products is
one of the possible approaches to reduce production costs. In this work, various industrial wastes were tested for laccase
production by Trametes versicolor MZKI G-99. Solid waste from chemomechanical treatment facility of a paper manufacturing plant showed the highest potential
for laccase production. Enzyme production during submerged cultivation of T. versicolor on the chosen industrial waste has been further improved by medium optimization using genetic algorithm. Concentrations of
five components in the medium were optimized within 60 shake-flasks experiments, where the highest laccase activity of 2,378 U dm−3 was achieved. Waste from the paper industry containing microparticles of CaCO3 was found to stimulate the formation of freely dispersed mycelium and laccase production during submerged cultivation of
T. versicolor. It was proven to be a safe and inexpensive substrate for commercial production of laccase and might be more widely applicable
for metabolite production by filamentous fungi. |
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