Isolation of thermo-stable and solvent-tolerant Bacillus sp. lipase for the production of biodiesel |
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Authors: | Sivaramakrishnan Ramachandran Muthukumar Karuppan |
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Institution: | (1) Department of Chemical Engineering, Alagappa College of Technology Campus, Anna University, Chennai, 600 025, India; |
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Abstract: | This study presents the production of biodiesel from algae oil by transesterification using thermophilic microorganism. The
microorganism used in this study was isolated from the soil sample obtained near the furnace. The organism was identified
as Bacillus sp., and the lipase obtained was purified by ammonium sulfate precipitation and ion exchange chromatography leading to 8.6-fold
purification and 13% recovery. Molecular weight of the enzyme was determined by sodium dodecyl sulfate-polyacrylamide gel
electrophoresis, and it was found to be 45 kDa. The effect of pH, temperature, and solvent addition on lipase activity was
investigated. The enzyme showed maximum activity at 55 °C and at pH 7 and was also found to be highly active in the presence
of organic solvents such as hexane and t-butanol. The isolated lipase was successfully used for the production of biodiesel. The transesterification activity of the
isolated lipase showed 76% of fatty acid methyl esters yield in 40 h, which indicated that this enzyme can be used as a potential
biocatalyst for the biodiesel production. |
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