A Thermodynamic Study of Zinc Ion Interaction with Bovine Carbonic Anhydrase II at Different Temperatures |
| |
| Authors: | L Barzegar G Rezaei Behbehani A A Saboury |
| |
| Institution: | (1) Chemistry Department, Imam Khomeini International University, Qazvin, Iran;(2) Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran;(3) School of Chemistry, University of Tehran, Tehran, Iran |
| |
| Abstract: | A thermodynamic study of the interaction of bovine carbonic anhydrase II, CAII, with zinc ion was carried out by using isothermal
titration calorimetry (ITC) at 300, 310 and 320 K in Tris buffer solutions at pH=7.5. The heats of Zn2++ CAII interaction are reported and analyzed in terms of the new solvation theory. It was indicated that there are three identical
and non-cooperative sites on CAII for Zn2+. The binding of a zinc ion is exothermic with dissociation equilibrium constants of 78.32, 95.81 and 116.70 mmol⋅L−1 at 300, 310 and 320 K respectively. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
