Entry of large nanoparticles into cells aided by nanoscale mechanical stimulation |
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Authors: | Ramanathan Vaidyanathan Adam Curtis Margaret Mullin |
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Institution: | (1) Center for Cell Engineering, University of Glasgow, Glasgow, G12 8QQ, UK |
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Abstract: | Nanoparticle entry into the cell depends on the surface charge and also on their size. Here, we report the entry of large
magnetic nanoparticles (500 nm mean diameter) into the cell, being mediated by a mechanical stimulus supplied to the culture
flasks. Investigations were carried out at 2–10 Hz frequency range with the vertical excursions ranging from 5 to 20 nm. Mechanical
stimulation was found to aid the entry of both positive and negatively charged nanoparticles over a frequency range of 2–10 Hz.
Transmission electron microscopy analysis indicated that, the stimulated samples could possibly mediate particle uptake through
membrane invaginations, while the control samples indicated particles at the cell periphery, just outside the cell membrane.
Mechanical stimulation had no significant effect on the cell morphology. Bromodeoxyuridine incorporation resulted in an increase
in the proportion of S-phase in the stimulated samples compared with the controls, suggesting a reduction in the cell cycle
duration. Mechanical stimulation could very well extend its effects to nanoscale cellular movements, and also facilitate the
entry of large magnetic nanoparticle. This could be an interesting prospect for nanoparticle mediated drug delivery. |
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