Determination of aflatoxin B1 in cereals by homogeneous liquid-liquid extraction coupled to high performance liquid chromatography-fluorescence detection |
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Authors: | Sheijooni-Fumani Neda Hassan Jalal Yousefi Seyed R |
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Affiliation: | International Goods Inspection Company, Tehran, Iran. n.sheijooni@yahoo.com |
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Abstract: | A simple and rapid method based on homogeneous liquid-liquid extraction coupled to HPLC with fluorescence detection was developed for the determination of aflatoxin B1 (AFB1) in the rice and grain samples after post-column derivatization. The proposed method eliminated the use of immunoaffinity columns for clean-up in the determination of AFB1. The parameters affecting recovery and preconcentration such as type and volume of organic solvent, volume ratio of water/methanol, concentration of phase separator reagent and extraction time were optimized. Under the optimized conditions, the calibration graph was linear in the concentration range of 0.01-1.0 ng/g with the detection limit of 0.003 ng/g. This method was successfully applied for the analysis of AFB1 in different cereal samples. |
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Keywords: | Aflatoxin B1 Homogenized liquid–liquid extraction HPLC‐fluorescence detection |
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