Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution |
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Authors: | Li Wei Herrman Timothy J Dai Susie Y |
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Affiliation: | Office of the Texas State Chemist, Texas AgriLife Research, Texas A&M University, College Station, TX 77843, USA. |
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Abstract: | The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid-phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra-day assay precision of 2% to 15% and an inter-day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds. |
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