荧光光度法研究金莲橙G与牛血清白蛋白的作用

利用荧光光度法研究了金莲橙G与牛血清白蛋白(BSA)的相互作用。金莲橙G对牛血清白蛋白的内源荧光有显著的猝灭作用,是形成超分子复合物的静态猝灭过程。测定了金莲橙G与BSA结合常数以及其相互作用的热力学参数,并根据Ross理论,和作用过程的热力学参数确定了金莲橙G与牛血清白蛋白之间主要结合力是静电引力。由Foerster非辐射能量转移理论,推导出能量供体金莲橙G与受体BSA间的结合距离。

Study on the Interaction Between Tropeolin G and Bovine Serum Albumin by Fluorescence Spectroscopy

The interaction between tropeolin G and bovine serum albumin（BSA）was investigated mainly by fluorescence spectroscopy.Tropeolin G can remarkably quench the fluorescence intensity of BSA,and the fluorescence quenching effect is a static quenching process with forming the supramolecular complex.The binding constants and thermodynamic parameters of interaction between tropeolin G and bovine serum albumin were studied.Basing on the theory of Ross,the main sort of binding force between tropeolin G and BSA were attributed to static-electricity gravitation,which was conformed by the calculation results of thermodynamic parameters of this process.The binding distance between tropeolin G and BSA was obtained according to Foerster＇s nonradioactive energy transfer theory.