Agmatine protects retinal ganglion cells from hypoxia-induced apoptosis in transformed rat retinal ganglion cell line |
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| Authors: | Samin Hong Jong Eun Lee Chan Yun Kim Gong Je Seong |
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| Institution: | (1) Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea;(2) Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea |
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| Abstract: | Background Agmatine is an endogenous polyamine formed by the decarboxylation of L-arginine. We investigated the protective effects of
agmatine against hypoxia-induced apoptosis of immortalized rat retinal ganglion cells (RGC-5). RGC-5 cells were cultured in
a closed hypoxic chamber (5% O2) with or without agmatine. Cell viability was determined by lactate dehydrogenase (LDH) assay and apoptosis was examined
by annexin V and caspase-3 assays. Expression and phosphorylation of mitogen-activated protein kinases (MAPKs; JNK, ERK p44/42,
and p38) and nuclear factor-kappa B (NF-κB) were investigated by Western immunoblot analysis. The effects of agmatine were
compared to those of brain-derived neurotrophic factor (BDNF), a well-known protective neurotrophin for retinal ganglion cells. |
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