Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers |
| |
Authors: | Shuye Jiang Qifeng Chen Xuanjun Fang |
| |
Institution: | (1) Biotechnology Research Center, Chinese Academy of Agricultural Science, 100081 Beijing, China;(2) Institute of Genetics and Crop Breeding, Fujian Agricultural University, 350002 Fuzhou, China |
| |
Abstract: | The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young
panicles in the photo-period sensitive stage of fertility transformation. Two populations were analyzed using cDNA-RAPD. The
results showed that: (i) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD,
taking an advantage in avoiding false positive caused by conventional sampling method. (II) Among 150 random primers used,
83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of
intensity on gel. (iii) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the
ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the
differentially expressed cDNA fragments complicated. |
| |
Keywords: | photo-period sensitive genic male sterile genes cDNA-RAPD differential display analysis |
本文献已被 SpringerLink 等数据库收录! |
|