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Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue |
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| Authors: | Kim Hwan Myung Jeong Byeong Ha Hyon Ju-Yong An Myoung Jin Seo Mun Sik Hong Jin Hee Lee Kyoung J Kim Chul Hoon Joo Taiha Hong Seok-Cheol Cho Bong Rae |
| Institution: | Department of Chemistry, National Creative Research Initiative Center for Cell Dynamics, Department of Physics, Biomicrosystems Technology Program, Korea University, 1-Anamdong, Seoul, 136-701, Korea. |
| Abstract: | We report a new two-photon fluorescence turn-on probe 6-(E)-3-oxo-1-dodecenyl]-2-N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy. |
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