| 古细菌RNase HII与金属离子结合的热力学研究 |
| |
| 引用本文： | 赖兵,李颖,曹傲能,来鲁华.古细菌RNase HII与金属离子结合的热力学研究[J].物理化学学报,2001,17(10):865-867. |
| |
| 作者姓名： | 赖兵 李颖 曹傲能 来鲁华 |
| |
| 作者单位： | State Key Laboratory for Structural Studies of Stable and Unstable Species,Institute of Physical Chemistry,College of Chemistry,Peking University,Beijing 100871 |
| |
| 基金项目： | 国家自然科学基金(29525306)及科技部资助项目 |
| |
| 摘 要： | RNase H是一种专一性水解RNA:DNA杂合链中RNA链的核糖核酸酶,它广泛存在于从原核生物到人的生物体中.本文通过等温滴定量热技术研究了Mg2＋,Mn2＋和Ca2＋与一种古细菌Methanococcus jannaschii中的II型RNase H结合的热力学.首次用这种方法获得了这一结合过程的热力学参数.并证实了这些金属离子与RNase HII按1∶1结合.为RNase HII酶反应机理和折叠研究提供了重要信息.
|
| 关 键 词： | RNase HII 等温滴定量热 二价金属离子 |
| 收稿时间： | 2001-06-11 |
| 修稿时间： | 2001年6月11日 |
Thermodynamic Study on Binding of Archaebacterial RNase H |
| |
| Lai Bing,Li Ying,Cao Ao-Neng,Lai Lu-Hua.Thermodynamic Study on Binding of Archaebacterial RNase H[J].Acta Physico-Chimica Sinica,2001,17(10):865-867. |
| |
| Authors: | Lai Bing Li Ying Cao Ao-Neng Lai Lu-Hua |
| |
| Institution: | State Key Laboratory for Structural Studies of Stable and Unstable Species,Institute of Physical Chemistry,College of Chemistry,Peking University,Beijing 100871 |
| |
| Abstract: | RNase H,a ribonuclease specifically degrades the RNA chain in RNA:DNA hybrid,exists vastly in organisms from procaryotic to humanic.We use isothermal titration calorimetry to study the binding thermodynamics of Mg2＋,Mn2＋,Ca2＋ to a type II RNase H from an archaeon:Methanococcus jannaschii.For the first time we obtain the thermodynamic parameters of this binding reaction and verify that these metal ions bind with RNase HII with a ratio of 1:1.This will give an important information for studying RNase HII reaction mechanism and folding properties. |
| |
| Keywords: | RNase HII Isothermal titration calorimetry Divalent metal ion |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《物理化学学报》浏览原始摘要信息 |
| 点击此处可从《物理化学学报》下载免费的PDF全文 |
