| 同位素标记结合中性丢失扫描鉴定白蛋白早期糖基化修饰肽段 |
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| 引用本文： | 苏锐,王云,刘亚辉,洪杰,张永谦,李玉娟,庆宏,邓玉林.同位素标记结合中性丢失扫描鉴定白蛋白早期糖基化修饰肽段[J].北京理工大学学报,2014,34(12):1310-1314. |
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| 作者姓名： | 苏锐 王云 刘亚辉 洪杰 张永谦 李玉娟 庆宏 邓玉林 |
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| 作者单位： | 北京理工大学生物医药成分分离与分析实验室,北京 100081;中北大学化工与环境学院,山西,太原030051;北京理工大学生物医药成分分离与分析实验室,北京 100081 |
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| 基金项目： | 国家"九七三"计划项目(2012CB910603;2012YQ040140-10) |
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| 摘 要： | 采用 13C6] 同位素标记的葡萄糖作为标签,在体外建立非酶糖基化反应模型,并采用MALDI-TOF对模型中的糖基化人血清白蛋白(HSA)进行验证. 以高分辨率的液质联用四级杆飞行时间质谱(HPLC/Q-TOF MS)为分析平台,经过一级质谱(LC/MS)筛选糖基化肽段. 采用target MS/MS模式对筛选出的34条肽段进行分析. 运用蛋白质组学数据分析工具pFind软件对肽段的中性丢失扫描及二级质谱(LC/MS/MS)数据进行分析,最终获得16条早期糖基化修饰肽段的序列及修饰位点信息.
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| 关 键 词： | 同位素标记 中性丢失 糖基化肽段 |
| 收稿时间： | 2013/6/21 0:00:00 |
Identification of Early Glycated Peptides of Albumin by a Method of Isotopic Labeling Combined with Neutral Loss Scan |
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| SU Rui,WANG Yun,LIU Ya-hui,HONG Jie,ZHANG Yong-qian,LI Yu-juan,QING Hong and DENG Yu-lin.Identification of Early Glycated Peptides of Albumin by a Method of Isotopic Labeling Combined with Neutral Loss Scan[J].Journal of Beijing Institute of Technology(Natural Science Edition),2014,34(12):1310-1314. |
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| Authors: | SU Rui WANG Yun LIU Ya-hui HONG Jie ZHANG Yong-qian LI Yu-juan QING Hong and DENG Yu-lin |
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| Institution: | 1.Beijing Key Laboratory of Separation and Analysis in Biomedicine and Pharmaceuticals, Beijing Institute of Technology, Beijing 100081, China;School of Chemical Engineering and Environment, North University of China, Taiyuan, Shanxi 030051, China2.Beijing Key Laboratory of Separation and Analysis in Biomedicine and Pharmaceuticals, Beijing Institute of Technology, Beijing 100081, China |
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| Abstract: | This project utilized isotopic labeling with 13C6] glucose as a tag, establishing non-enzymatic glycation reaction model in vitro. Glycated the model human serum albumin (HSA) was verified using MALDI-TOF. The glycated peptides from the digestion HSA were selected by LC/MS on a high resolution HPLC/Quadrupole time of flight mass (HPLC/Q-TOF MS) analysis platform. The 34 screened glycated peptides were analyzed using target MS/MS mode. The pFind software, which is a proteomics data analysis tool, was applied for the automated data analysis of the neutral scan and LC/MS/MS data. Finally, this method allowed to obtain the information of sequences and modified site of 16 early glycated peptides. |
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| Keywords: | isotopic label neutral loss glycated peptides |
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