Expression and regulation of orphan receptor TR2 mRNA in germ cells of cryptorchid testis in rat and rhesus monkey |
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Authors: | Caixia Guo Zhaoyuan Hu Rujin Zou Xiaomin Mu Yixun Liu |
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Institution: | (1) State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China;(2) Kunming Institute of Zoology, Chinese Academy of Sciences, 650223 Kunming, China |
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Abstract: | Expression and cellular localization of orphan receptor TR2 mRNA in relation to germ cell apoptosis in cryptorchid testes
of rat and rhesus monkey have been studied by usingin situ hybridization andin situ 3′-end labeling of DNA fragments (TUNEL). The results show that: (i) TR2 mRNA is specifically expressed in the germ cells,
mainly in the spermatocytes, round and elongated spermatids. The expression level of TR2 mRNA varies with the seminiferous
cycle, (ii) In the rat cryptorchid testes on days 3 and 5 after the surgery, the germ cells began to undergo apoptosis with
no evident decrease in TR2 mRNA level. On day 7.5, however, most germ cells underwent apoptosis, while the expression level
of TR2 mRNA declined markedly, and TR2 mRNA was rarely expressed on day 10 thereafter, (iii) On days 15 and 20 of the cryptorchid
testes of rhesus monkey, TR2 mRNA was only expressed in a few of primary spermatocytes and the mRNA was almost undetectable
on days 30, 45, 60. These results suggest that TR2 mRNA probably plays an important role in spermatogenesis and germ cell
apoptosis. |
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Keywords: | orphan receptor TR2 in situ hybridization cryptorchidism spermatogenesis apoptosis |
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