超高效液相色谱串联质谱快速测定饮用水中9种N-亚硝胺的新方法

N-亚硝胺是潜在的人类致癌物,是近年来关注的一类饮用水消毒副产物,同时也是环境分析研究的热点.本文建立了超高效液相色谱(UPLC)串联质谱快速测定饮用水中9种N-亚硝胺的新方法,讨论了色谱柱和流动相对分离9种N-亚硝胺的影响,优化了多级反应质谱(MRM,MS/MS)条件.二甲基亚硝胺-d6为贮存和回收率内标,亚硝基二丙...

Rapid and sensitive method for simultaneous determination of nine N-nitrosamines in drinking water by UPLC-MS/MS

N-Nitrosomines, a group of probable human carcinogens, has been detected as a disinfection by-products (DBPs) in drinking water. To comprehensively investigate the occurrence of possible nitrosamines in drinking water, an ultra-performance liquid chromatography-tandem mass spectrometry technique was developed to detect both thermally stable and unstable total nine nitrosamines. With optimized liquid chromatography column, mobile phases, and triple quadrupole conditions, UPLC-MS/MS enabled the determination of ng/L levels of nine nitrosamines. Isotope-labeled N-nitrosodimethylamine-d6 (NDMA-d6) was used as the surrogate standard for determining recovery, and N-nitrosodi-n-propylamine-d14 (NDPA-d14) was used as the internal standard for quantification. The limits of detection (LOD) and limits of quantification (LOQ) for NDMA were estimated to be 0.1 and 1 ng/L, NDPA were 2.5 and 10 ng/L, and other seven nitrosamines were 0.5 and 2.0 ng/L, respectively, which were less than maximum permissible concentration by US EPA. The occurrence and distribution of N-nitrosamines in different treatment processes (raw water, deposition and finished water) in drinking water treatment plants with chloramines disinfectant were determined using UPLC-MS/MS with direct injection method. The three nitrosamines with carcinogen risk, NDMA is found in finished water with concentration 3.5 ng/L, NMEA and NDEA were lower than LOQ. This indicated N-nitrosamines in drinking water that could not lead to the health risk for urban residents.