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Simultaneous determination of creatine and creatinine using amperometric biosensors |
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| Authors: | Stefan Raluca-Ioana Bokretsion Rahel Girmai van Staden Jacobus F Aboul-Enein Hassan Y |
| Affiliation: | a Department of Chemistry, University of Pretoria, 0002, Pretoria, South Africa b Pharmaceutical Analysis and Drug Development Laboratory, Biological and Medical Research Department (MBC-03-65), King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia |
| Abstract: | In order to determine creatine and creatinine amperometric biosensors were proposed. A bienzymatic biosensor based on creatinase (CI) and sarcosine oxidase (SO) was used for the assay of creatine and a trienzymatic biosensor based on CI, SO and creatininase (CA) for the assay of creatinine. The linear concentration ranges are of pmol l−1 to nmol l−1 magnitude order, with very low limits of detection. The biosensors proved high reliability for determination of creatine and creatinine as raw material, and in the pharmaceutical formulation. |
| Keywords: | Creatine Creatinine Amperometric biosensors Creatininase Creatinase Sorcosine oxidase |
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