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Simultaneous determination of creatine and creatinine using amperometric biosensors |
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| Authors: | Stefan Raluca-Ioana Bokretsion Rahel Girmai van Staden Jacobus F Aboul-Enein Hassan Y |
| Institution: | a Department of Chemistry, University of Pretoria, 0002, Pretoria, South Africa b Pharmaceutical Analysis and Drug Development Laboratory, Biological and Medical Research Department (MBC-03-65), King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia |
| Abstract: | In order to determine creatine and creatinine amperometric biosensors were proposed. A bienzymatic biosensor based on creatinase (CI) and sarcosine oxidase (SO) was used for the assay of creatine and a trienzymatic biosensor based on CI, SO and creatininase (CA) for the assay of creatinine. The linear concentration ranges are of pmol l?1 to nmol l?1 magnitude order, with very low limits of detection. The biosensors proved high reliability for determination of creatine and creatinine as raw material, and in the pharmaceutical formulation. |
| Keywords: | Creatine Creatinine Amperometric biosensors Creatininase Creatinase Sorcosine oxidase |
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