Selenium methylselenocysteine protects human hepatoma HepG2 cells against oxidative stress induced by tert-butyl hydroperoxide |
| |
Authors: | Susana Cuello Sonia Ramos Raquel Mateos M Angeles Martín Yolanda Madrid Carmen Cámara Laura Bravo Luis Goya |
| |
Institution: | (1) Departamento de Química Analítica, Facultad de Químicas, Universidad Complutense, 28040 Madrid, Spain;(2) Departamento de Metabolismo y Nutrición, Instituto del Frío (CSIC), José Antonio Novais 10, 28040 Madrid, Spain |
| |
Abstract: | Selenium methylselenocysteine (Se-MeSeCys) is a common selenocompound in the diet with a tested chemopreventive effect. This
study investigated the potential protective effect of Se-MeSeCys against a chemical oxidative stress induced by tert-butyl hydroperoxide (t-BOOH) on human hepatoma HepG2 cells. Speciation of selenium derivatives by liquid chromatography–inductively
coupled plasma mass spectrometry depicts Se-MeSeCys as the only selenocompound in the cell culture. Cell viability (lactate
dehydrogenase) and markers of oxidative status—concentration of reduced glutathione (GSH) and malondialdehyde (MDA), generation
of reactive oxygen species (ROS) and activity of the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase
(GR)—were evaluated. Pretreatment of cells with Se-MeSeCys for 20 h completely prevented the enhanced cell damage, MDA concentration
and GR and GPx activity and the decreased GSH induced by t-BOOH but did not prevent increased ROS generation. The results
show that treatment of HepG2 cells with concentrations of Se-MeSeCys in the nanomolar to micromolar range confers a significant
protection against an oxidative insult. |
| |
Keywords: | Antioxidant defences Biomarkers for oxidative stress Dietary antioxidants Selenium compounds |
本文献已被 PubMed SpringerLink 等数据库收录! |