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Chemical Synthesis of the 12 kDa Human Myokine Irisin by α‐Ketoacid‐Hydroxylamine (KAHA) Ligation |
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| Authors: | Thomas G Wucherpfennig Sebastian Müller Christian Wolfrum Jeffrey W Bode |
| Institution: | 1. Laboratory for Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich;2. Laboratory of Translational Nutrition Biology, Department of Health Sciences and Technology, ETH Zurich, Schwerzenbach;3. Institute of Transformative Bio‐Molecules (WPI‐ITbM), Nagoya University, Chikusa, Nagoya, Japan |
| Abstract: | Irisin is a recently discovered protein hormone with a conserved sequence among vertebrates and with putative functions in the regulation of adipose tissue and bone metabolism. We report the first chemical synthesis using two sequential ketoacid‐hydroxylamine (KAHA) ligations to give milligram quantities of unlabeled and fluorescence‐labeled irisin protein. The synthetic proteins were utilized in cell binding assays, which indicated the expected binding characteristics to stromal cells of white adipose tissue. These studies strongly imply the presence of a specific irisin receptor and provide a path to its identification with synthetic irisin. |
| Keywords: | Proteins Cytokines Total synthesis Metabolism Ligation Reactions |