LIGHT-INDUCED FOURIER TRANSFORM INFRARED SPECTROSCOPIC INVESTIGATIONS OF THE INTERMEDIARY ELECTRON ACCEPTOR REDUTION IN BACTERIAL PHOTOSYNTHESIS |
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Authors: | E Nabedryk W Mäntele B A Tavitian J Breton |
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Institution: | Service de Biophysique, Département de Biologie, C. E. N. Saclay, 91191 Gif-sur-Yvette Cédex, France;Institut für Biophysik und Strahlenbiologie der Universität Freiburg, Albertstrasse 23, D-7800 Freiburg, W. Germany |
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Abstract: | Abstract— Molecular changes associated with the light-induced reduction of the intermediary electron acceptor I (bacteriopheophytin, BPh) in bacterial photosynthesis were studied by means of Fourier transform infrared (FTIR) difference spectroscopy. Chromatophore membranes and reconstituted reaction centers (RCs) of Rhodopseudomonas viridis were prereduced with sodium dithionite and illuminated in order to trap photochemically the state I−. Fourier transform infrared spectra of these samples were recorded before, during and after illumination, with an accuracy better than 10−3 absorbance units. Difference spectra of I− in chromatophores and in RCs closely correspond to each other. In the carbonyl stretching frequency region between 1640 and 1750 cm−1, bands are tentatively attributed to a shift (from 1713 to 1683 cm−1) of a keto carbonyl group, a change of an acetyl carbonyl grou at 1656 cm−1 and a decrease in absorbance strength of ester carbonyl groups (at 1746 and 1732 cm−P) after reduction of I. These groups likely belong to the BPh molecule, although at least one of the ester carbonyls could be assigned to an amino acid side chain. The absence of strong bands in the amide I and amide II region excludes large protein conformational changes associated with I reduction. |
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