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Dynamic visualization of membrane-inserted fraction of pHluorin-tagged channels using repetitive acidification technique |
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| Authors: | Serguei S Khiroug Evgeny Pryazhnikov Sarah K Coleman Andreas Jeromin Kari Kein?nen Leonard Khiroug |
| Institution: | (1) Neuroscience Center, University of Helsinki, FIN-00014 Helsinki, Finland;(2) Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, FIN-00014 Helsinki, Finland;(3) Allen Institute for Brain Science, Seattle, WA 98103, USA |
| Abstract: | BackgroundChanges in neuronal excitability, synaptic efficacy and generally in cell signaling often result from insertion of key molecules into plasma membrane (PM). Many of the techniques used for monitoring PM insertion lack either spatial or temporal resolution. |
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