Development of an iridium-based pH sensor for bioanalytical applications |
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| Authors: | S Bause M Decker F Gerlach J Näther F Köster P Neubauer W Vonau |
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| Institution: | 1.Kurt-Schwabe-Institut für Mess- und Sensortechnik e.V. Meinsberg,Waldheim,Germany;2.Technische Universit?t Berlin, Chair of Bioprocess Engineering,Berlin,Germany;3.Hochschule Mittweida, Fachgruppe Fertigungs- und Werkstofftechnik,University of Applied Sciences,Mittweida,Germany |
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| Abstract: | A new iridium-based planar pH sensor for bioanalytical purposes is introduced. The fabrication of the sensor was carried out by a two-stage coating process of different iridium solutions on a platinum thick film surface. The pH response behaviour and the Nernstian characteristics of the double-layer electrode exhibited better results than the single iridium depositions. An almost theoretical Nernstian slope could be obtained as well as a pH response time of about 3 to 5 min in a pH range of 4.01 to 9.18. Furthermore, a biofilm growth of different microorganisms onto the iridium-coated electrodes could be achieved. Afterwards, the viability of the microorganisms was demonstrated via cell plating studies. |
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