Properties of DNA from Various Local Strains of Bradyrhizobium sp. (Vigna radiata) |
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| Authors: | S S Muradova T Yu Yusupov I M Khalilov A G Guzalova M M Muradov |
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| Institution: | (1) Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent |
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| Abstract: | The buoyant density of DNA from various local strains of mung-bean nodule bacteria (Bradyrhizobium sp.) was determined by ultracentrifugation in an equilibrium CsCl gradient. The number of GC base pairs (61.1-62.7% mol %) and DNA melting point (94.3-95.0°C) were calculated from the buoyant density. DNA preparations purified by ultracentrifugation in a CsCl gradient by electrophoresis show a bimodal distribution. The DNA composition varies little (1.6 mol %). The electrophoretic distribution of local and Type-strain mung-bean nodule bacteria are identical. This proves that they belong to a single taxonomic group. |
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| Keywords: | nodule bacteria Bradyrhizobium sp taxonomy DNA |
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